International FISH Course

The International FISH Course 2019: September 9 – 13, Vienna, Austria
This course will provide an introduction to the fundamentals of fluorescence in situ hybridization (FISH)-based microscopy for identification, quantification, and co-localization of microorganisms and outline complementary techniques for in situ analysis. Practical work focuses (i) on the different basic aspects of FISH with rRNA-targeted oligonucleotide probes and (ii) on FISH analyses of the participants own samples.

The course includes seminars on:

  • Principles of fluorescence in situ hybridization
  • Application of FISH in microbial ecology
  • Confocal laser scanning microscopy (CLSM)
  • Computer-assisted image analysis
  • Probe design and evaluation
  • Advanced FISH techniques [e.g. CARD-FISH, DOPE-FISH, Clone-FISH, FISH-MAR, Raman-FISH, NanoSIMS-FISH]

Laboratory course: The main focus is practical work with own samples.
It is our mission to provide theoretical advice and practical help for FISH [CARD-FISH, DOPE-FISH] with the participants own samples.

All participants are asked to:

  • bring their own samples (instructions on preservation of samples will be provided)
  • prepare a short presentation on their own research interests (PowerPoint, approximately 5 minutes)

Course fee: € 2000,- [members of non-profit organizations such as universities receive a 50% discount!]
The fee includes the official course dinner on Tuesday and coffee/tea during the course but not other meals and accommodation. Payment only via bank transfer.

The course takes place at:

Division of Microbial Ecology
Department of Microbiology & Ecosystem Science
Universität Wien
Althanstr. 14
A-1090 Wien
Phone: +43 1 4277 76601

To apply, please fill out the application form and submit it by email to until May 17.

Notification of acceptance is on May 24.

We ask you for understanding that there is a limited number of participants for the course and participants will thus be selected based on the submitted abstracts.



The International FISH course is supported by